Effect of Tumor Relevant Acidic Environment in the Interaction of a N-hydroxyindole-2-Carboxylic Derivative with the Phospholipid Bilayer. Please enable it to take advantage of the complete set of features! Campbell RB, Balasubramanian SV, Straubinger RM. In order to investigate the relationship between lipid structure and liposome-mediated gene transfer, we have studied biophysical parameters and transfection properties of monocationic DOTAP analogs, systematically modified in their non-polar hydrocarbon chains. We are further showing that, in mixed DOTAP-DPPC systems, the reduction of the phase transition temperature is accompanied by a reduction of the average size of the structures present in the aqueous mixtures, whatever the DOTAP concentration is. Cholesterol-containing lipoplexes were generally stable, showing high transfection efficiency at low relative fluidity. Acta 2001, 27, 1512.) Eur Phys J E Soft Matter. Copyright © 2020 Elsevier B.V. or its licensors or contributors. We find that the incorporation of increasing quantities of DOTAP progressively reduces the temperature and the enthalpy of the gel-to-liquid crystalline transition. Biochim Biophys Acta. When anisotropy levels are high ( r >0.2; Fig. USA.gov. Get the latest research from NIH: https://www.nih.gov/coronavirus. When antimony is deposited at room temperature on bismuth selenide, it forms domains of α-antimonene having different orientations with respect to the substrate. | Get the latest public health information from CDC: https://www.coronavirus.gov. | Thus, the heat capacity C p at the transition point is infinite. Phase Transition Temperature Phase transition temperatures determined by DSC measurements were 25.3°C (enthalpy change, ΔΗ =34.1kJmol-1) and 29.1°C (ΔΗ =32.7kJmol-1) for aqueous vesicles of 1 and 2, respectively. National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. These results, which extend a previous investigation by Campbell et al. 2001 May 2;1512(1):27-39. doi: 10.1016/s0005-2736(01)00290-5. We report the discovery of a temperature-induced phase transition between the α and β structures of antimonene. In addition, the electronic friction has been introduced as a constant offset μ el, which vanishes well below the phase transition temperature T c. On the basis of this model, the relation between friction coefficient, temperature, and velocity above T c can be written as μ (v, T) = μ ph + μ el = [α − β T 2 / 3 (ln (γ T v) ) 2 / 3] + μ el 2008 Feb 15;61(2):304-10. doi: 10.1016/j.colsurfb.2007.09.010. Chem Phys Lipids. Above the phase transition temperature correct? Clipboard, Search History, and several other advanced features are temporarily unavailable. The Effect of Phase Transition Temperature on Therapeutic Efficacy of Liposomal Bortezomib Anticancer Agents Med Chem. In copolymeric systems, the swelling process can be controlled by the introduction of the appropriate amount of a second monomer with hydrophobic and hydrophilic characters. NIH 2020;20(6):700-708. doi: … From: Studies in Surface Science and Catalysis, 2001 Low Temperature Structural Phase Transition in the Perovskite Ba 2 CaMoO 6 Loi T. Nguyen,1,2 Robert J. Cava,2 Allyson M. Fry-Petit1* 1Department of Chemistry and Biochemistry, California State University, Fullerton, California 92831, United States 2 Department of Chemistry, Princeton University, Princeton, New Jersey 08544, United States *Corresponding author Stability, size and (by means of anisotropy profiles) membrane fluidity of liposomes and lipoplexes were determined, and lipofection efficiency was tested in a luciferase reporter gene assay. We use cookies to help provide and enhance our service and tailor content and ads. | Biochimica et Biophysica Acta (BBA) - Biomembranes, https://doi.org/10.1016/S0005-2736(00)00126-7. Reprinted with permission from John Wiley & Sons, Inc. Lipid Thermotropic Phase Transition Database (LIPIDAT) – NIST Standard Reference Database 34 2014 Jan;177:71-90. doi: 10.1016/j.chemphyslip.2013.11.008. The measurement of fluorescence anisotropy is an appropriate method to characterize membrane fluidity within a defined system of liposomes or lipoplexes and may be helpful to elucidate structure–activity relationships. This site needs JavaScript to work properly. Epub 2007 Sep 11. Biosphys. Thermotropic Phase Transitions of Pure Lipids in Model Membranes and Their Modifications by Membrane Proteins, Dr. John R. Silvius, Lipid-Protein Interactions, John Wiley & Sons, Inc., New York, 1982. Stability of liposomes was a precondition for formation of temporarily stable lipoplexes. A DSC and FTIR spectroscopic study of the effects of the epimeric 4-cholesten-3-ols and 4-cholesten-3-one on the thermotropic phase behaviour and organization of dipalmitoylphosphatidylcholine bilayer membranes: comparison with their 5-cholesten analogues. 2013 Jul;36(7):77. doi: 10.1140/epje/i2013-13077-0. Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. The phase transition temperature is defined as the temperature required to induce a change in the lipid physical state from the ordered gel phase, where the hydrocarbon chains are fully extended and closely packed, to the disordered liquid crystalline phase, where … HHS 2A ), i.e. Inclusion of DOPE led to rather uniform transfection and anisotropy profiles, corresponding to lipoplex stability. Czolkos I, Erkan Y, Dommersnes P, Jesorka A, Orwar O. Nano Lett. 2009;19(3):220-30. doi: 10.1080/08982100902746596. Our results demonstrate that the efficiency of gene transfer mediated by monocationic lipids is greatly influenced by lipoplex biophysics due to lipid composition. Calorimetric study of the interaction of binary DMTAP/DOTAP cationic liposomes with plasmid DNA. Phospholipid-cationic lipid interactions: influences on membrane and vesicle properties. These results, which extend a previous investigation by Campbell et al. Benesch MG, Mannock DA, Lewis RN, McElhaney RN. Addition of DOPE or cholesterol improved liposome and lipoplex stability. The transition temperature can be altered by chemical interaction, e.g., via copolymerization, as the physical nature of the volume phase transition [13–16]. Pharm Res. 2011 Feb 16;100(4):875-84. doi: 10.1016/j.bpj.2010.11.049. NLM We have investigated the effect of a cationic lipid [DOTAP] on both the thermotropic phase behavior and the structural organization of aqueous dispersions of dipalmitoyl-phosphatidylcholine [DPPC] by means of high-sensitivity differential scanning calorimetry and dynamic light scattering measurements. I want to ensure best formation of SUV’s from the LMV’s and LUV’s. Liposomes composed of pure DOTAP analogs display characteristic temperature–anisotropy curves (or profiles) and in all cases, except for DSTAP, typical phase transition can be seen. (Campbell, R. B.; Balasubramanian, S. V.; Straubinger, R. M.; Biochim. Interaction of diverse voltage sensor homologs with lipid bilayers revealed by self-assembly simulations. According to the measurements, photoanodes with 3% of SiO 2 result in improved efficiency, which is mainly related to increased surface area and dye loading. 2018 Jul 9;35(9):175. doi: 10.1007/s11095-018-2449-7. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. Biophysical and lipofection studies of DOTAP analogs, HPTLC, high-performance thin-layer chromatography. In chemistry, thermodynamics, and many other related fields, phase transitions (or phase changes) are the physical processes of transition between the basic states of matter: solid, liquid, and gas, as well as plasma in rare cases. membranes show rigid character, there can also be found unstable liposomes. X-ray diffraction and Raman spectroscopy data show that anatase-to-rutile phase transition temperature is delayed by the presence of SiO 2, enabling single-anatase phase photoanodes for DSSCs. Giatrellis S, Nikolopoulos G, Sideratou Z, Nounesis G. J Liposome Res. The temperature associated with this reduction in the intensity of the detected light is representative of the phase transition temperature of the sample. Monti D, Tampucci S, Zucchetti E, Granchi C, Minutolo F, Piras AM. Transfection efficiencies of lipoplexes based on pure DOTAP analogs could be correlated with stability data and membrane fluidity at transfection temperature. This self-assembling restructuring from an aqueous multilamellar structure toward a liposomal phase is favored by decreasing the phospholipid phase transition temperature and by increasing the temperature of the system. Advisor: Dr. Sarah Keller Student: Jialing Li Phase Transition in Lipid Bilayers Ethanol’s Effect on Miscibility Transition Temperature in Ternary Vesicles COVID-19 is an emerging, rapidly evolving situation. Copyright © 2000 Elsevier Science B.V. All rights reserved. Controlled formation and mixing of two-dimensional fluids. Influence of charge density on bilayer bending rigidity in lipid vesicles: a combined dynamic light scattering and neutron spin-echo study. This reduction of the average size and the appearance of a stable liposomal phase is also promoted by a heating and cooling thermal treatment.
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